Fig. 1
Experimental protocol. Rats were exposed to carbon disulfide (CS2) and noise for 6 h/day, 5 days/week, over 4 weeks. The pink noise level was 106 dB SPL (LEX,8h = 105 dB SPL) and the spectrum was a band-pass filtered over three octave bands centered at 0.5, 1 and 2 kHz. CS2 exposure was continuous (63 or 250 ppm) or intermittent (1 × 15 min/h or 2 × 15 min/h at 250 ppm CS2). Distortion product otoacoustic emissions were used to test hearing prior to exposure (T0: DPOAE0), following the exposure period (T1: DPOAE1), and after a 4-week recovery post-exposure period (T2: DPOAE2). Vestibular function was assessed using post-rotary nystagmus measurements prior to exposure (T0: PRN0), at the end of the exposure period (T1: PRN1), and after the 4-week recovery period (T2: PRN2). Blood and urine samples were collected at T1. Histological analyses were performed at T1 and T2